New potential markers for the detection of boldenone misuse.Both the increases in speed and resolution make this an attractive method; boldenone z testosteronem to My library Help Advanced Book Search. Royal Society of Chemistry- Science - pages. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than papers have been written on this strongly-evolving topic to testoxteronem. This book provides a solid background boldenone z testosteronem how to work with UHPLC and its application to the life sciences.
New potential markers for the detection of boldenone misuse. - PubMed - NCBI
Boldenone is one of the most frequently detected anabolic androgenic steroids in doping control analysis. However, some cases of endogenous boldenone and BM1 have been reported. Nowadays, when these compounds are detected in urine at low concentrations, isotope ratio mass spectrometry IRMS analysis is needed to confirm their exogenous origin.
The aim of the present study was to identify boldenone metabolites conjugated with sulphate and to evaluate their potential to improve the detection of boldenone misuse in sports. Boldenone was administered to a healthy volunteer and urine samples were collected up to 56h after administration. Boldenone sulphate was identified in the excretion study urine samples and, moreover, another peak with the same transition was observed.
In order to verify the usefulness of these new metabolites to discriminate between endogenous and exogenous origin of boldenone, four samples containing endogenous boldenone and BM1, confirmed by IRMS, were analysed. In 3 of the 4 samples, neither boldenone sulphate nor epiboldenone sulphate were detected, confirming that these metabolites were mainly detected after exogenous administration of boldenone.
In contrast, boldenone sulphate and, in some cases, epiboldenone sulphate were present in samples with low concentrations of exogenous boldenone and BM1. Thus, boldenone and epiboldenone sulphates are additional markers for the exogenous origin of boldenone and they can be used to reduce the number of samples to be analysed by IRMS. In samples with boldenone and BM1 at the concentrations suspicion for endogenous origin, only if boldenone and epiboldenone sulphates are present, further analysis by IRMS will be needed to confirm exogenous origin.
National Center for Biotechnology Information , U. Didn't get the message? Add to My Bibliography. Generate a file for use with external citation management software. J Steroid Biochem Mol Biol. Epub Jun 2. Abstract Boldenone is one of the most frequently detected anabolic androgenic steroids in doping control analysis.